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<t>qRT‐PCR</t> <t>validation</t> of gene expression changes related to cognitive function. (A–E, G, H, K, L) The Lyz2 (Ordinary one‐way ANOVA, F (2,27) = 102.0, p < 0.0001), Cd74 (Ordinary one‐way ANOVA, F (2,27) = 60.91, p < 0.0001), H2‐Eb1 (Ordinary one‐way ANOVA, F (2,27) = 136.9, p < 0.0001), Slc11a1 (Ordinary one‐way ANOVA, F (2,27) = 40.07, p < 0.0001), H2‐K1 (Ordinary one‐way ANOVA, F (2,27) = 90.56, p < 0.0001), Mid1 (Ordinary one‐way ANOVA, F (2,27) = 61.00, p < 0.0001), Gins2 (Ordinary one‐way ANOVA, F (2,27) = 65.38, p < 0.0001), Des (Ordinary one‐way ANOVA, F (2,27) = 26.82, p < 0.0001), Hba‐a1 (Ordinary one‐way ANOVA, F (2,27) = 35.63, p < 0.0001) genes were up‐regulated at 28d after UL and down‐regulated by aerobic exercise. (F, I, J) The Clec1a (Ordinary one‐way ANOVA, F (2,27) = 17.63, p = 0.0002), Cntn5 (Ordinary one‐way ANOVA, F (2,27) = 50.28, p < 0.0001), Glp2R (Ordinary one‐way ANOVA, F (2,27) = 58.78, p < 0.0001) genes were down‐regulated at 28d after UL and up‐regulated by aerobic exercise ( n = 10/group in qRT‐PCR).
Chamq Sybr Qrt Pcr Master Mix, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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<t>qRT‐PCR</t> <t>validation</t> of gene expression changes related to cognitive function. (A–E, G, H, K, L) The Lyz2 (Ordinary one‐way ANOVA, F (2,27) = 102.0, p < 0.0001), Cd74 (Ordinary one‐way ANOVA, F (2,27) = 60.91, p < 0.0001), H2‐Eb1 (Ordinary one‐way ANOVA, F (2,27) = 136.9, p < 0.0001), Slc11a1 (Ordinary one‐way ANOVA, F (2,27) = 40.07, p < 0.0001), H2‐K1 (Ordinary one‐way ANOVA, F (2,27) = 90.56, p < 0.0001), Mid1 (Ordinary one‐way ANOVA, F (2,27) = 61.00, p < 0.0001), Gins2 (Ordinary one‐way ANOVA, F (2,27) = 65.38, p < 0.0001), Des (Ordinary one‐way ANOVA, F (2,27) = 26.82, p < 0.0001), Hba‐a1 (Ordinary one‐way ANOVA, F (2,27) = 35.63, p < 0.0001) genes were up‐regulated at 28d after UL and down‐regulated by aerobic exercise. (F, I, J) The Clec1a (Ordinary one‐way ANOVA, F (2,27) = 17.63, p = 0.0002), Cntn5 (Ordinary one‐way ANOVA, F (2,27) = 50.28, p < 0.0001), Glp2R (Ordinary one‐way ANOVA, F (2,27) = 58.78, p < 0.0001) genes were down‐regulated at 28d after UL and up‐regulated by aerobic exercise ( n = 10/group in qRT‐PCR).
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<t>qRT‐PCR</t> <t>validation</t> of gene expression changes related to cognitive function. (A–E, G, H, K, L) The Lyz2 (Ordinary one‐way ANOVA, F (2,27) = 102.0, p < 0.0001), Cd74 (Ordinary one‐way ANOVA, F (2,27) = 60.91, p < 0.0001), H2‐Eb1 (Ordinary one‐way ANOVA, F (2,27) = 136.9, p < 0.0001), Slc11a1 (Ordinary one‐way ANOVA, F (2,27) = 40.07, p < 0.0001), H2‐K1 (Ordinary one‐way ANOVA, F (2,27) = 90.56, p < 0.0001), Mid1 (Ordinary one‐way ANOVA, F (2,27) = 61.00, p < 0.0001), Gins2 (Ordinary one‐way ANOVA, F (2,27) = 65.38, p < 0.0001), Des (Ordinary one‐way ANOVA, F (2,27) = 26.82, p < 0.0001), Hba‐a1 (Ordinary one‐way ANOVA, F (2,27) = 35.63, p < 0.0001) genes were up‐regulated at 28d after UL and down‐regulated by aerobic exercise. (F, I, J) The Clec1a (Ordinary one‐way ANOVA, F (2,27) = 17.63, p = 0.0002), Cntn5 (Ordinary one‐way ANOVA, F (2,27) = 50.28, p < 0.0001), Glp2R (Ordinary one‐way ANOVA, F (2,27) = 58.78, p < 0.0001) genes were down‐regulated at 28d after UL and up‐regulated by aerobic exercise ( n = 10/group in qRT‐PCR).
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<t>qRT‐PCR</t> <t>validation</t> of gene expression changes related to cognitive function. (A–E, G, H, K, L) The Lyz2 (Ordinary one‐way ANOVA, F (2,27) = 102.0, p < 0.0001), Cd74 (Ordinary one‐way ANOVA, F (2,27) = 60.91, p < 0.0001), H2‐Eb1 (Ordinary one‐way ANOVA, F (2,27) = 136.9, p < 0.0001), Slc11a1 (Ordinary one‐way ANOVA, F (2,27) = 40.07, p < 0.0001), H2‐K1 (Ordinary one‐way ANOVA, F (2,27) = 90.56, p < 0.0001), Mid1 (Ordinary one‐way ANOVA, F (2,27) = 61.00, p < 0.0001), Gins2 (Ordinary one‐way ANOVA, F (2,27) = 65.38, p < 0.0001), Des (Ordinary one‐way ANOVA, F (2,27) = 26.82, p < 0.0001), Hba‐a1 (Ordinary one‐way ANOVA, F (2,27) = 35.63, p < 0.0001) genes were up‐regulated at 28d after UL and down‐regulated by aerobic exercise. (F, I, J) The Clec1a (Ordinary one‐way ANOVA, F (2,27) = 17.63, p = 0.0002), Cntn5 (Ordinary one‐way ANOVA, F (2,27) = 50.28, p < 0.0001), Glp2R (Ordinary one‐way ANOVA, F (2,27) = 58.78, p < 0.0001) genes were down‐regulated at 28d after UL and up‐regulated by aerobic exercise ( n = 10/group in qRT‐PCR).
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<t>qRT‐PCR</t> <t>validation</t> of gene expression changes related to cognitive function. (A–E, G, H, K, L) The Lyz2 (Ordinary one‐way ANOVA, F (2,27) = 102.0, p < 0.0001), Cd74 (Ordinary one‐way ANOVA, F (2,27) = 60.91, p < 0.0001), H2‐Eb1 (Ordinary one‐way ANOVA, F (2,27) = 136.9, p < 0.0001), Slc11a1 (Ordinary one‐way ANOVA, F (2,27) = 40.07, p < 0.0001), H2‐K1 (Ordinary one‐way ANOVA, F (2,27) = 90.56, p < 0.0001), Mid1 (Ordinary one‐way ANOVA, F (2,27) = 61.00, p < 0.0001), Gins2 (Ordinary one‐way ANOVA, F (2,27) = 65.38, p < 0.0001), Des (Ordinary one‐way ANOVA, F (2,27) = 26.82, p < 0.0001), Hba‐a1 (Ordinary one‐way ANOVA, F (2,27) = 35.63, p < 0.0001) genes were up‐regulated at 28d after UL and down‐regulated by aerobic exercise. (F, I, J) The Clec1a (Ordinary one‐way ANOVA, F (2,27) = 17.63, p = 0.0002), Cntn5 (Ordinary one‐way ANOVA, F (2,27) = 50.28, p < 0.0001), Glp2R (Ordinary one‐way ANOVA, F (2,27) = 58.78, p < 0.0001) genes were down‐regulated at 28d after UL and up‐regulated by aerobic exercise ( n = 10/group in qRT‐PCR).
Quantitative Real Time Pcr Qrt Pcr, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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<t>qRT‐PCR</t> <t>validation</t> of gene expression changes related to cognitive function. (A–E, G, H, K, L) The Lyz2 (Ordinary one‐way ANOVA, F (2,27) = 102.0, p < 0.0001), Cd74 (Ordinary one‐way ANOVA, F (2,27) = 60.91, p < 0.0001), H2‐Eb1 (Ordinary one‐way ANOVA, F (2,27) = 136.9, p < 0.0001), Slc11a1 (Ordinary one‐way ANOVA, F (2,27) = 40.07, p < 0.0001), H2‐K1 (Ordinary one‐way ANOVA, F (2,27) = 90.56, p < 0.0001), Mid1 (Ordinary one‐way ANOVA, F (2,27) = 61.00, p < 0.0001), Gins2 (Ordinary one‐way ANOVA, F (2,27) = 65.38, p < 0.0001), Des (Ordinary one‐way ANOVA, F (2,27) = 26.82, p < 0.0001), Hba‐a1 (Ordinary one‐way ANOVA, F (2,27) = 35.63, p < 0.0001) genes were up‐regulated at 28d after UL and down‐regulated by aerobic exercise. (F, I, J) The Clec1a (Ordinary one‐way ANOVA, F (2,27) = 17.63, p = 0.0002), Cntn5 (Ordinary one‐way ANOVA, F (2,27) = 50.28, p < 0.0001), Glp2R (Ordinary one‐way ANOVA, F (2,27) = 58.78, p < 0.0001) genes were down‐regulated at 28d after UL and up‐regulated by aerobic exercise ( n = 10/group in qRT‐PCR).
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<t>qRT‐PCR</t> <t>validation</t> of gene expression changes related to cognitive function. (A–E, G, H, K, L) The Lyz2 (Ordinary one‐way ANOVA, F (2,27) = 102.0, p < 0.0001), Cd74 (Ordinary one‐way ANOVA, F (2,27) = 60.91, p < 0.0001), H2‐Eb1 (Ordinary one‐way ANOVA, F (2,27) = 136.9, p < 0.0001), Slc11a1 (Ordinary one‐way ANOVA, F (2,27) = 40.07, p < 0.0001), H2‐K1 (Ordinary one‐way ANOVA, F (2,27) = 90.56, p < 0.0001), Mid1 (Ordinary one‐way ANOVA, F (2,27) = 61.00, p < 0.0001), Gins2 (Ordinary one‐way ANOVA, F (2,27) = 65.38, p < 0.0001), Des (Ordinary one‐way ANOVA, F (2,27) = 26.82, p < 0.0001), Hba‐a1 (Ordinary one‐way ANOVA, F (2,27) = 35.63, p < 0.0001) genes were up‐regulated at 28d after UL and down‐regulated by aerobic exercise. (F, I, J) The Clec1a (Ordinary one‐way ANOVA, F (2,27) = 17.63, p = 0.0002), Cntn5 (Ordinary one‐way ANOVA, F (2,27) = 50.28, p < 0.0001), Glp2R (Ordinary one‐way ANOVA, F (2,27) = 58.78, p < 0.0001) genes were down‐regulated at 28d after UL and up‐regulated by aerobic exercise ( n = 10/group in qRT‐PCR).
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<t>qRT‐PCR</t> <t>validation</t> of gene expression changes related to cognitive function. (A–E, G, H, K, L) The Lyz2 (Ordinary one‐way ANOVA, F (2,27) = 102.0, p < 0.0001), Cd74 (Ordinary one‐way ANOVA, F (2,27) = 60.91, p < 0.0001), H2‐Eb1 (Ordinary one‐way ANOVA, F (2,27) = 136.9, p < 0.0001), Slc11a1 (Ordinary one‐way ANOVA, F (2,27) = 40.07, p < 0.0001), H2‐K1 (Ordinary one‐way ANOVA, F (2,27) = 90.56, p < 0.0001), Mid1 (Ordinary one‐way ANOVA, F (2,27) = 61.00, p < 0.0001), Gins2 (Ordinary one‐way ANOVA, F (2,27) = 65.38, p < 0.0001), Des (Ordinary one‐way ANOVA, F (2,27) = 26.82, p < 0.0001), Hba‐a1 (Ordinary one‐way ANOVA, F (2,27) = 35.63, p < 0.0001) genes were up‐regulated at 28d after UL and down‐regulated by aerobic exercise. (F, I, J) The Clec1a (Ordinary one‐way ANOVA, F (2,27) = 17.63, p = 0.0002), Cntn5 (Ordinary one‐way ANOVA, F (2,27) = 50.28, p < 0.0001), Glp2R (Ordinary one‐way ANOVA, F (2,27) = 58.78, p < 0.0001) genes were down‐regulated at 28d after UL and up‐regulated by aerobic exercise ( n = 10/group in qRT‐PCR).
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qRT‐PCR validation of gene expression changes related to cognitive function. (A–E, G, H, K, L) The Lyz2 (Ordinary one‐way ANOVA, F (2,27) = 102.0, p < 0.0001), Cd74 (Ordinary one‐way ANOVA, F (2,27) = 60.91, p < 0.0001), H2‐Eb1 (Ordinary one‐way ANOVA, F (2,27) = 136.9, p < 0.0001), Slc11a1 (Ordinary one‐way ANOVA, F (2,27) = 40.07, p < 0.0001), H2‐K1 (Ordinary one‐way ANOVA, F (2,27) = 90.56, p < 0.0001), Mid1 (Ordinary one‐way ANOVA, F (2,27) = 61.00, p < 0.0001), Gins2 (Ordinary one‐way ANOVA, F (2,27) = 65.38, p < 0.0001), Des (Ordinary one‐way ANOVA, F (2,27) = 26.82, p < 0.0001), Hba‐a1 (Ordinary one‐way ANOVA, F (2,27) = 35.63, p < 0.0001) genes were up‐regulated at 28d after UL and down‐regulated by aerobic exercise. (F, I, J) The Clec1a (Ordinary one‐way ANOVA, F (2,27) = 17.63, p = 0.0002), Cntn5 (Ordinary one‐way ANOVA, F (2,27) = 50.28, p < 0.0001), Glp2R (Ordinary one‐way ANOVA, F (2,27) = 58.78, p < 0.0001) genes were down‐regulated at 28d after UL and up‐regulated by aerobic exercise ( n = 10/group in qRT‐PCR).

Journal: CNS Neuroscience & Therapeutics

Article Title: Aerobic Exercise Promotes Hippocampal Neurogenesis and Ameliorates Cognitive Dysfunction Induced by Unilateral Labyrinthectomy

doi: 10.1002/cns.70773

Figure Lengend Snippet: qRT‐PCR validation of gene expression changes related to cognitive function. (A–E, G, H, K, L) The Lyz2 (Ordinary one‐way ANOVA, F (2,27) = 102.0, p < 0.0001), Cd74 (Ordinary one‐way ANOVA, F (2,27) = 60.91, p < 0.0001), H2‐Eb1 (Ordinary one‐way ANOVA, F (2,27) = 136.9, p < 0.0001), Slc11a1 (Ordinary one‐way ANOVA, F (2,27) = 40.07, p < 0.0001), H2‐K1 (Ordinary one‐way ANOVA, F (2,27) = 90.56, p < 0.0001), Mid1 (Ordinary one‐way ANOVA, F (2,27) = 61.00, p < 0.0001), Gins2 (Ordinary one‐way ANOVA, F (2,27) = 65.38, p < 0.0001), Des (Ordinary one‐way ANOVA, F (2,27) = 26.82, p < 0.0001), Hba‐a1 (Ordinary one‐way ANOVA, F (2,27) = 35.63, p < 0.0001) genes were up‐regulated at 28d after UL and down‐regulated by aerobic exercise. (F, I, J) The Clec1a (Ordinary one‐way ANOVA, F (2,27) = 17.63, p = 0.0002), Cntn5 (Ordinary one‐way ANOVA, F (2,27) = 50.28, p < 0.0001), Glp2R (Ordinary one‐way ANOVA, F (2,27) = 58.78, p < 0.0001) genes were down‐regulated at 28d after UL and up‐regulated by aerobic exercise ( n = 10/group in qRT‐PCR).

Article Snippet: One microgram of RNA was reverse transcribed to complementary DNA (cDNA) using HiScript II Q RT SuperMix for quantitative reverse transcription PCR (qRT‐PCR) with a genomic DNA (gDNA) wiper ( R22301 , Vazyme, Nanjing, China). qRT‐PCR was performed on a BioRad CFX384 Real‐Time System using ChamQ SYBR qRT‐PCR Master Mix (Q311‐02, Vazyme, Nanjing, China) according to the manufacturer's protocol.

Techniques: Quantitative RT-PCR, Biomarker Discovery, Gene Expression